Co-Aggregation and Parallel Aggregation of Specific Proteins in Major Mental Illness

Background: Disrupted proteostasis is an emerging area of research into major depressive disorder. Several proteins have been implicated as forming aggregates specifically in the brains subsets patients with psychiatric illnesses. These include CRMP1, DISC1, NPAS3 and TRIOBP-1. It unclear, however, whether these normally aggregate together same individuals and, if so, each protein independently other (“parallel aggregation”) or physically interact (“co-aggregation”). Materials methods: Post mortem insular cortex samples from disorder Alzheimer’s disease patients, suicide victims control had their insoluble fractions isolated tested by Western blotting to determine which are therefore, likely be aggregating. The ability co-aggregate (directly form common structures) was systematic pairwise expression SH-SY5Y neuroblastoma cells, were then examined immunofluorescent microscopy. Results: Many displayed multiple brain, although not enough imply interaction between proteins. Cell culture analysis revealed that only a few analyzed can consistently other: DISC1 CRMP1 able induce aggregation full length TRIOBP-1, but individual domains TRIOBP-1 when they expressed individually. Conclusions: While specific capable co-aggregating, appear do so mental illness potentially also suicidal tendency, it more for such parallel manner, through independent mechanisms. This information aids understanding distribution among therefore important any future diagnostic therapeutic approaches based on this aspect pathology.